修饰核苷在转运核糖核酸中的作用：位于酵母赖氨酸转运核糖核酸2反密码子5′端的2-硫代尿苷衍生物修饰的影响
Role of modified nucleosides in tRNA: effect of modification of the 2-thiouridine derivative located at the 5'-end of the anticodon of yeast transfer RNA Lys2.
作者信息
Sen G C, Ghosh H P
出版信息
Nucleic Acids Res. 1976 Mar;3(3):523-35. doi: 10.1093/nar/3.3.523.
Abstract
Yeast tRNA Lys2 codes preferentially for AAA and contains a 2-thiouridine derivative (U) at the 5'-position of the anticodon. Removal of the 2-thio group from U by treatment with CNBr did not affect the amino acid accepting activity of the modified tRNA Lys2. CNBr treated tRNA Lys2 was active in protein synthesis but with a much reduced efficiency. Although the modified tRNA Lys2 was recognized by elongation factor (EF) T, the EFT dependent binding to ribosomes to tRNA Lys2 (CNBr) was markedly decreased.
摘要
酵母tRNA Lys2优先编码AAA，并且在反密码子的5'-位置含有2-硫尿苷衍生物（U）。用溴化氰处理从U上去除2-硫基并不影响修饰后的tRNA Lys2的氨基酸接受活性。经溴化氰处理的tRNA Lys2在蛋白质合成中具有活性，但效率大大降低。尽管修饰后的tRNA Lys2被延伸因子（EF）T识别，但EF-T依赖的与核糖体结合到tRNA Lys2（溴化氰处理）上的情况明显减少。
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本文引用的文献
1
Nucleotide sequence of a lysine transfer ribonucleic Acid from bakers' yeast.从酿酒酵母中分离出的赖氨酸转移核糖核酸的核苷酸序列。
Science. 1972 May 12;176(4035):687-9. doi: 10.1126/science.176.4035.687.
2
Primary sequence of glutamic acid tRNA II from Escherichia coli.来自大肠杆菌的谷氨酸tRNA II的一级序列。
FEBS Lett. 1972 Feb 1;20(2):239-241. doi: 10.1016/0014-5793(72)80804-4.
3
5-methyl-2-thiouridine: A new sulfur-containing minor constituent from rat liver glutamic acid and lysine tRNAs.5-甲基-2-硫代尿苷：一种来自大鼠肝脏谷氨酸和赖氨酸转运RNA的新型含硫微量成分。
FEBS Lett. 1971 Apr 2;13(6):335-338. doi: 10.1016/0014-5793(71)80254-5.
4
Codon--anticodon pairing: the wobble hypothesis.密码子-反密码子配对：摆动假说
J Mol Biol. 1966 Aug;19(2):548-55. doi: 10.1016/s0022-2836(66)80022-0.
5
Structural studies on a yeast glutamic acid tRNA specific to GAA codon.对一种特异识别GAA密码子的酵母谷氨酸转运核糖核酸的结构研究。
Biochim Biophys Acta. 1971 Jan 1;228(1):153-66. doi: 10.1016/0005-2787(71)90555-7.
6
Selective inactivation of amino acid acceptor and ribosome-binding activities of Escherichia coli tRNA by modification with cyanogen bromide.用溴化氰修饰对大肠杆菌tRNA的氨基酸受体和核糖体结合活性进行选择性失活。
Biochim Biophys Acta. 1971 Aug 12;246(1):123-31. doi: 10.1016/0005-2787(71)90077-3.
7
Presumed anticodon structure of glutamic acid tRNA from E. coli: a possible location of a 2-thiouridine derivative in the first position of the anticodon.来自大肠杆菌的谷氨酸转运RNA的假定反密码子结构：反密码子第一位上2-硫代尿苷衍生物的可能位置。
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8
Specificity of yeast glutamic acid transfer RNA for codon recognition.酵母谷氨酸转运RNA对密码子识别的特异性。
Biochim Biophys Acta. 1969 Jun 17;182(2):411-26. doi: 10.1016/0005-2787(69)90192-0.
9
Chemical probe of structure and function of transfer ribonucleic acids.转运核糖核酸结构与功能的化学探针
Biochemistry. 1974 Jul 2;13(14):2924-32. doi: 10.1021/bi00711a023.
10
Oligonucleotide composition of a yeast lysine transfer ribonucleic acid.酵母赖氨酸转移核糖核酸的寡核苷酸组成
Biochemistry. 1974 Jan 29;13(3):518-23. doi: 10.1021/bi00700a018.
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