Gavella M, Lipovac V, Sverko V
Vuk Vrhovac Institute, University Clinic for Diabetes, Endocrinology and Metabolic Diseases, Medical Faculty University of Zagreb, Croatia.
Andrologia. 1995 Jan-Feb;27(1):7-12. doi: 10.1111/j.1439-0272.1995.tb02088.x.
The relationship between superoxide anion production and the activities of sperm specific NADH-diaphorase and isozyme LDH-C4 has been investigated in 25 normozoospermic and 17 oligozoospermic semen samples. The samples were separated into high and low density sperm populations on discontinuous, two step (40%:80%) Percoll gradients prior to analysis. Superoxide anion generation was significantly higher (P < 0.001) and diaphorase activity significantly increased (P < 0.01) in both low and high density sperm fractions from oligozoospermic patients compared to the values found in normozoospermic men. A significant positive correlation (P < 0.01) between diaphorase activity and superoxide anion production was established in the oligozoospermic samples of low density sperm fraction. A tendency of statistically insignificant increase in LDH-C4 isozyme activity was observed in the oligozoospermic samples. The activity of this isozyme appears to have no association with superoxide anion generation or diaphorase activity. These findings seem to suggest that excessive superoxide anion production along with increased diaphorase activity point to biochemical abnormalities which may affect the functional status of sperms. Hence, determination of these parameters may provide useful data in male infertility of unknown etiology.
在25份正常精子精液样本和17份少精子症精液样本中，研究了超氧阴离子产生与精子特异性NADH - 黄递酶和同工酶LDH - C4活性之间的关系。在分析之前，通过不连续的两步（40%:80%）Percoll梯度将样本分离为高密度和低密度精子群体。与正常精子男性相比，少精子症患者的低密度和高密度精子部分中超氧阴离子生成显著更高（P < 0.001），黄递酶活性显著增加（P < 0.01）。在低密度精子部分的少精子症样本中，黄递酶活性与超氧阴离子产生之间建立了显著的正相关（P < 0.01）。在少精子症样本中观察到LDH - C4同工酶活性有统计学上无显著意义的增加趋势。该同工酶的活性似乎与超氧阴离子生成或黄递酶活性无关。这些发现似乎表明，过量的超氧阴离子产生以及增加的黄递酶活性表明可能影响精子功能状态的生化异常。因此，这些参数的测定可能为不明病因的男性不育症提供有用的数据。
