“可溶性脂质”在生化过程中的应用：水溶液中的亚油酸 - 环糊精包合物
Use of 'soluble lipids' for biochemical processes: linoleic acid-cyclodextrin inclusion complexes in aqueous solutions.
作者信息
López-Nicolás J M, Bru R, Sánchez-Ferrer A, García-Carmona F
机构信息
Departamento de Bioquímica y Biología Molecular A, Facultad de Biología, Universidad de Murcia, Spain.
出版信息
Biochem J. 1995 May 15;308 ( Pt 1)(Pt 1):151-4. doi: 10.1042/bj3080151.
Abstract
The equilibria of linoleic acid (LA)-cyclodextrin (CD) complexes were studied to investigate the behaviour of 'soluble lipids' in solution as a function of factors that typically affect biochemical processes, such as pH, temperature and CD structure. The above complexes are formed with a stoicheiometry of 1:2 in solution. The first CD molecule interacts with LA through hydrogen bonds when the pH is below the fatty acid pK; hydrophobic interactions may also play an important role at high pH. The second CD molecule makes only hydrophobic contact with the LA hydrocarbon chain. The formation of hydrogen bonds is dependent on the inner diameter of the CD whereas the strength of the hydrophobic interactions between CD and LA can be related to the presence of hydrophobic groups in the CD. The first CD molecule interacts more strongly with LA at increased temperatures. The quantitative description of the LA-CD interaction allows absolute control of the effects produced by the lipid on biochemical processes.
摘要
研究了亚油酸（LA）-环糊精（CD）复合物的平衡，以研究溶液中“可溶性脂质”的行为与通常影响生化过程的因素（如pH值、温度和CD结构）之间的关系。上述复合物在溶液中的化学计量比为1:2。当pH值低于脂肪酸的pK时，第一个CD分子通过氢键与LA相互作用；在高pH值下，疏水相互作用也可能起重要作用。第二个CD分子仅与LA烃链进行疏水接触。氢键的形成取决于CD的内径，而CD与LA之间疏水相互作用的强度可能与CD中疏水基团的存在有关。第一个CD分子在温度升高时与LA的相互作用更强。LA-CD相互作用的定量描述使得能够绝对控制脂质对生化过程产生的影响。
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