Nagata R, Yokoyama K, Clark S A, Karube I
Central Research Institute, Dai Nippon Printing Co., Ltd., Chiba, Japan.
Biosens Bioelectron. 1995;10(3-4):261-7. doi: 10.1016/0956-5663(95)96845-p.
A novel glucose sensor employing ferrocene-modified glucose oxidase is fabricated using the screen printing technique. Glucose oxidase is covalently bound to the electron mediator ferrocenecarboxylic acid in order to obtain higher enzyme activity. The ferrocene-glucose oxidase shows an increased catalytic current because the ferrocene acts as an electron transfer relay between the active centre of the enzyme and the gold electrode. Glucose sensors employing enzymes modified with ferrocene in various ways are successfully fabricated using the screen printing technique. The ink component containing the ferrocene-glucose oxidase is specially developed to be applicable to the printing machine. The printed glucose sensor chip offers a stable calibration profile and stable electrochemical properties.
采用丝网印刷技术制备了一种新型的基于二茂铁修饰葡萄糖氧化酶的葡萄糖传感器。葡萄糖氧化酶与电子媒介体二茂铁羧酸共价结合，以获得更高的酶活性。二茂铁修饰的葡萄糖氧化酶显示出增加的催化电流，因为二茂铁在酶的活性中心和金电极之间起到电子传递中继的作用。采用丝网印刷技术成功制备了多种以不同方式用二茂铁修饰酶的葡萄糖传感器。含有二茂铁修饰葡萄糖氧化酶的油墨成分经过专门研发，适用于印刷机。印刷的葡萄糖传感器芯片具有稳定的校准曲线和稳定的电化学性能。
