Solomon B O, Zeng A P, Biebl H, Schlieker H, Posten C, Deckwer W D
GBF-Gesellschaft für Biotechnologische Forschung mbH, Biochemical Engineering Division, Braunschweig, Germany.
J Biotechnol. 1995 Apr 15;39(2):107-17. doi: 10.1016/0168-1656(94)00148-6.
Data for the anaerobic growth of Klebsiella pneumoniae DSM 2026 and Clostridium butyricum DSM 5431 on glycerol have been analyzed using the concept of material and available electron balances with consideration for hydrogen production. Models for the kinetics of energetic efficiencies of product formation under low residual glycerol are presented. For Klebsiella pneumoniae, the specific rates of electron transfer to the products were mainly significantly dependent on specific growth rate with the exception of ethanol and hydrogen which were also significantly non-growth associated. In the case of Clostridium butyricum, the rates were only growth rate dependent, except for hydrogen formation. The analysis also indicated that the production of 1,3-propanediol by Klebsiella pneumoniae was favoured by limitations other than glycerol limitation, while hydrogen generation was best under low residual glycerol and particularly in the presence of external 1,3-propanediol. Klebsiella pneumoniae appeared to be able to incorporate more of the available electrons of glycerol into hydrogen as compared with the Clostridium butyricum. The study demonstrates the need for properly considering H2 in models describing anaerobic processes.
已使用物质和可用电子平衡的概念，并考虑氢气产生，对肺炎克雷伯菌DSM 2026和丁酸梭菌DSM 5431在甘油上的厌氧生长数据进行了分析。给出了低残留甘油条件下产物形成能量效率动力学的模型。对于肺炎克雷伯菌，除乙醇和氢气外，电子向产物的比转移速率主要显著依赖于比生长速率，乙醇和氢气也显著与生长无关。对于丁酸梭菌，除氢气生成外，速率仅依赖于生长速率。分析还表明，除甘油限制外的其他限制条件有利于肺炎克雷伯菌生产1,3 - 丙二醇，而在低残留甘油条件下，特别是存在外部1,3 - 丙二醇时，氢气生成最佳。与丁酸梭菌相比，肺炎克雷伯菌似乎能够将更多甘油的可用电子纳入氢气中。该研究表明在描述厌氧过程的模型中需要适当考虑H2。
