Abbad-Andaloussi S, Amine J, Gerard P, Petitdemange H
Laboratoire de Chimie Biologique I, Université Henri Poincare, Nancy, Vandoeuvre-lès-Nancy, France.
J Appl Microbiol. 1998 Apr;84(4):515-22. doi: 10.1046/j.1365-2672.1998.00374.x.
The levels of 1,3-propanediol dehydrogenase and of the glycerol dehydrogenase in Clostridium butyricum grown on glucose-glycerol mixtures were similar to those found in extracts of cells grown on glycerol alone, which can explain the simultaneous glucose-glycerol consumption. On glycerol, 43% of glycerol was oxidized to organic acids to obtain energy for growth and 57% to produce 1,3-propanediol. With glucose-glycerol mixtures, glucose catabolism was used by the cells to produce energy through the acetate-butyrate production and NADH, whereas glycerol was used chiefly in the utilization of the reducing power since 92-93% of the glycerol flow was converted through the 1,3-propanediol pathway. The apparent K(m)s for the glycerol dehydrogenase was 16-fold higher for the glycerol than that for the glyceraldehyde in the case of the glyceraldehyde-3-phosphate dehydrogenase and fourfold higher for the NAD+, providing an explanation for the shift of the glycerol flow toward 1,3-propanediol when cells were grown on glucose-glycerol mixtures.
在以葡萄糖 - 甘油混合物为培养基生长的丁酸梭菌中，1,3 - 丙二醇脱氢酶和甘油脱氢酶的水平与仅以甘油为培养基生长的细胞提取物中的水平相似，这可以解释葡萄糖 - 甘油的同时消耗现象。在甘油培养基上，43%的甘油被氧化为有机酸以获取生长所需能量，57%用于产生1,3 - 丙二醇。对于葡萄糖 - 甘油混合物，细胞通过乙酸 - 丁酸生成和NADH利用葡萄糖分解代谢来产生能量，而甘油主要用于利用还原力，因为92 - 93%的甘油流通过1,3 - 丙二醇途径转化。在甘油醛 - 3 - 磷酸脱氢酶的情况下，甘油脱氢酶对甘油的表观K(m)值比对甘油醛的表观K(m)值高16倍，对NAD + 的表观K(m)值高4倍，这为细胞在葡萄糖 - 甘油混合物上生长时甘油流转向1,3 - 丙二醇提供了解释。
