Küpcü S, Sára M, Sleytr U B
Zentrum für Ultrastrukturforschung, Universität für Bodenkultur, Wien, Austria.
Biochim Biophys Acta. 1995 May 4;1235(2):263-9. doi: 10.1016/0005-2736(95)80013-6.
Isolated subunits from the crystalline cell surface layer (S-layer) of Bacillus coagulans E38-66 were recrystallized on positively charged liposomes. The liposomes were composed of dipalmitoylphosphatidylcholine/cholesterol and stearylamine. The natural arrangement of the S-layer subunits on the bacterial surface is as an oblique (p2) lattice. The subunits attached to positively charged liposomes by their inner face (which bears a net negative charge) in an orientation identical to the lattice on intact cells. The S-layer protein, once recrystallized on liposomes, was crosslinked with glutaraldehyde and subsequently used as a matrix for the covalent attachment of macromolecules. The high stability of S-layer-coated liposomes and the possibility for immobilizing biologically active molecules on the crystalline array may offer potential in various different liposome applications.
凝结芽孢杆菌E38 - 66晶体细胞表面层（S层）的分离亚基在带正电荷的脂质体上重结晶。脂质体由二棕榈酰磷脂酰胆碱/胆固醇和硬脂胺组成。S层亚基在细菌表面的自然排列为斜交（p2）晶格。亚基通过其内表面（带有净负电荷）以与完整细胞上晶格相同的方向附着在带正电荷的脂质体上。S层蛋白一旦在脂质体上重结晶，就用戊二醛交联，随后用作大分子共价连接的基质。S层包被脂质体的高稳定性以及将生物活性分子固定在晶体阵列上的可能性可能在各种不同的脂质体应用中具有潜力。
