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培养中的人胰岛：向培养基中添加同源和异源血清的影响。

Human pancreatic islets in culture: effects of supplementing the medium with homologous and heterologous serum.

作者信息

Goldman H, Colle E

出版信息

Science. 1976 Jun 4;192(4243):1014-6. doi: 10.1126/science.775637.

DOI:10.1126/science.775637
PMID:775637
Abstract

Islets of Langerhans, isolated from the human fetal pancreas and cultured in media supplemented with serums from human umbilical cord or adult donors, remained intact, free-floating, and functional for 2 to 5 months. Substitution of fetal calf serum for the human serum usually resulted in attachment of islets, monolayer formation, and relatively early (3 weeks) functional arrest.

摘要

从人胎儿胰腺分离出的胰岛，在补充了人脐带血清或成年供体血清的培养基中培养，可保持完整、漂浮状态并在2至5个月内保持功能。用人血清替代胎牛血清通常会导致胰岛附着、单层形成以及相对较早（3周）的功能停滞。

相似文献

1
Human pancreatic islets in culture: effects of supplementing the medium with homologous and heterologous serum.培养中的人胰岛：向培养基中添加同源和异源血清的影响。
Science. 1976 Jun 4;192(4243):1014-6. doi: 10.1126/science.775637.
2
Long-term effect of pH on B-cell function in isolated islets of Langerhans in tissue culture.pH对组织培养中分离的胰岛B细胞功能的长期影响。
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Preservation of beta cell function in adult human pancreatic islets for several months in vitro.成人人类胰岛β细胞功能在体外保存数月。
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[Monolayer culture of pancreatic beta cells of newborn rats: Insulin secretion in vitro and attempt at beta-cell transplantation in experimental diabetes].[新生大鼠胰腺β细胞单层培养：体外胰岛素分泌及实验性糖尿病中β细胞移植的尝试]
Probl Endokrinol (Mosk). 1978 Jul-Aug;24(4):57-60.
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Beta-cell function in isolated human pancreatic islets in long-term tissue culture.长期组织培养中分离的人胰岛的β细胞功能。
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Improved functional survival of human islets of Langerhans in three-dimensional matrix culture.三维基质培养中人类胰岛功能存活的改善
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Tissue culture of fetal rat islets: comparison of serum-supplemented and serum-free, defined medium on the maintenance, growth, and differentiation of A, B, and D cells.胎鼠胰岛的组织培养：在含血清和无血清限定培养基中对A、B和D细胞的维持、生长及分化的比较
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A new culture method for human pancreatic islets using a biopore membrane insert.一种使用生物孔膜插入物的人胰岛新培养方法。
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Emiocytosis of B granules from saurian pancreatic islets perifused in vitro.体外灌流的蜥蜴胰腺胰岛B颗粒的胞吐作用。
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Sequential differentiation of human fetal pancreas in vitro and in normal and diabetic nude mice.人胎儿胰腺在体外以及正常和糖尿病裸鼠体内的顺序分化
Transplant Proc. 1989 Feb;21(1 Pt 3):2657-8.

引用本文的文献

1
Islet cell surface antibodies in type 1 (insulin-dependent) diabetes mellitus: use of human fetal pancreas cultures as substrate.1型（胰岛素依赖型）糖尿病中的胰岛细胞表面抗体：以人胎胰培养物为底物的应用
Diabetologia. 1982 Feb;22(2):89-95. doi: 10.1007/BF00254835.
2
Spontaneous diabetes mellitus syndrome in the rat. I. Association with the major histocompatibility complex.大鼠自发性糖尿病综合征。I. 与主要组织相容性复合体的关联
J Exp Med. 1981 Oct 1;154(4):1237-42. doi: 10.1084/jem.154.4.1237.
3
Organ culture of the Islets of Langerhans from young and senescent rats.
来自幼年和老年大鼠的胰岛器官培养。
Cell Tissue Res. 1980;207(3):429-48. doi: 10.1007/BF00224618.
4
Pancreas and islet transplantation.
Jpn J Surg. 1982;12(6):391-404. doi: 10.1007/BF02469827.
5
Pancreas and islet transplantation. II. Clinical trials.胰腺与胰岛移植。II. 临床试验。
Diabetologia. 1981 Apr;20(4):435-50. doi: 10.1007/BF00253405.
6
Cell culture and in vitro studies of fresh and cryopreserved human insulinoma.新鲜和冷冻保存的人胰岛素瘤的细胞培养及体外研究。
In Vitro. 1982 Oct;18(10):884-90. doi: 10.1007/BF02796330.
7
Nonenzymic in vitro isolation of perinatal islets of Langerhans.围产期胰岛的非酶体外分离
In Vitro. 1983 Aug;19(8):611-20. doi: 10.1007/BF02619574.
8
Appearance of peroxidase reactivity within the rough endoplasmic reticulum of blood monocytes after surface adherence.血液单核细胞表面黏附后，粗面内质网内过氧化物酶反应性的出现。
J Exp Med. 1977 Feb 1;145(2):264-74. doi: 10.1084/jem.145.2.264.
9
Pancreas and islet transplantation.胰腺和胰岛移植。
World J Surg. 1977 Mar;2(1):185-95. doi: 10.1007/BF01665079.
10
Development in vitro of epithelial-cell monolayers derived from fetal rat pancreas.
In Vitro. 1979 Apr;15(4):270-7. doi: 10.1007/BF02618951.