Bodel P T, Nichols B A, Bainton D F
J Exp Med. 1977 Feb 1;145(2):264-74. doi: 10.1084/jem.145.2.264.
Rabbit blood monocytes, which contain no cytochemically demonstrable peroxidase, develop peroxidatic activity in the RER and perinuclear cisternae within 2 h after adherence to serum- or fibrin-coated surfaces. A similar reactivity appears in surface-adherent human and rat blood monocytes. In both localization and characteristics, this enzyme reactivity in monocytes resembles that normally seen in the resident peritoneal macrophages of the rabbit, as well as in several types of tissue macrophages in other species. Thus this observation supports the concept, presently based on the kinetic data of other investigators, that blood monocytes are the precursors of such cells. Moreover, the appearance of new enzyme activity after adherence may reflect alterations in cellular metabolism resulting from plasma membrane:surface interactions.
兔血单核细胞不含细胞化学可显示的过氧化物酶,在黏附于血清或纤维蛋白包被的表面后2小时内,其粗面内质网和核周池会产生过氧化物酶活性。表面黏附的人血和大鼠血单核细胞也出现类似反应。单核细胞中的这种酶反应性在定位和特征上与兔常驻腹膜巨噬细胞以及其他物种的几种组织巨噬细胞中通常所见的相似。因此,这一观察结果支持了目前基于其他研究者动力学数据的概念,即血单核细胞是这类细胞的前体。此外,黏附后新酶活性的出现可能反映了质膜与表面相互作用导致的细胞代谢变化。