Vaudequin-Dransart V, Petit A, Poncet C, Ponsonnet C, Nesme X, Jones J B, Bouzar H, Chilton W S, Dessaux Y
Institut des Sciences Végétales, CNRS, Gif-sur-Yvette, France.
Mol Plant Microbe Interact. 1995 Mar-Apr;8(2):311-21. doi: 10.1094/mpmi-8-0311.
Galls naturally induced on Fig and chrysanthemum plants by strains of Agrobacterium contained, in addition to other well-characterized opines such as nopaline, three tumor-specific opinelike molecules. These molecules were identified as deoxy-fructosyl-glutamine (dfg), deoxy-fructosyl-5-oxo-proline (dfop), and chrysopine (Chilton et al., unpublished). Strains isolated from Fig tree and chrysanthemum tumors harbored different and unrelated Ti plasmids as judged by hybridization with various vir and T-DNA probes. They also exhibited different opine-catabolic properties. The strains isolated from chrysanthemum plants (Chry strains) and Fig trees degraded chrysopine, but only the Chry strains used dfg and dfop. Remarkably, other strains of Agrobacterium catabolized these two molecules: dfg was degraded by most pathogenic and nonpathogenic Agrobacterium strains, and dfop by all Agrobacterium strains degrading the opine agropinic acid. These results have strong ecological and evolutionary inferences which fit previous speculation on the origin of opine-related functions.
根癌土壤杆菌菌株在无花果和菊花植株上自然诱导形成的瘿瘤，除了含有诸如胭脂碱等其他特征明确的冠瘿碱外，还含有三种肿瘤特异性的类冠瘿碱分子。这些分子被鉴定为脱氧果糖基谷氨酰胺（dfg）、脱氧果糖基 - 5 - 氧代脯氨酸（dfop）和菊花碱（奇尔顿等人，未发表）。通过与各种vir和T - DNA探针杂交判断，从无花果和菊花肿瘤中分离出的菌株携带不同且不相关的Ti质粒。它们还表现出不同的冠瘿碱分解代谢特性。从菊花植株（Chry菌株）和无花果中分离出的菌株能降解菊花碱，但只有Chry菌株利用dfg和dfop。值得注意的是，其他根癌土壤杆菌菌株能分解这两种分子：大多数致病和非致病的根癌土壤杆菌菌株能降解dfg，而所有能降解农杆碱型冠瘿碱的根癌土壤杆菌菌株都能降解dfop。这些结果具有重要的生态和进化推论，与先前关于冠瘿碱相关功能起源的推测相符。
