采用高效液相色谱-蒸发光散射检测法测定伏马毒素B1、B2、B3和B4

Determination of fumonisins B1, B2, B3 and B4 by high-performance liquid chromatography with evaporative light-scattering detection.

作者信息

Wilkes J G, Sutherland J B, Churchwell M I, Williams A J

机构信息

Division of Chemistry, National Center for Toxicological Research, United States Food and Drug Administration, Jefferson, AR 72079, USA.

出版信息

J Chromatogr A. 1995 Mar 31;695(2):319-23. doi: 10.1016/0021-9673(95)00064-t.

DOI:10.1016/0021-9673(95)00064-t

PMID:7757206

Abstract

Fumonisins B1, B2, B3 and B4 (FB1-FB4), a group of mycotoxins produced by the fungus Fusarium moniliforme, were separated by HPLC using an analytical-scale, base-deactivated C8 column and a gradient of trifluoroacetic acid buffer (pH 2.7) and acetonitrile. An evaporative light-scattering detector was used to detect the fumonisin peaks. A semi-preparative-scale, base-deactivated C8 column with a 1:14 mobile phase split facilitated the purification of analytical standards of FB.

摘要

伏马菌素B1、B2、B3和B4（FB1-FB4）是由串珠镰刀菌产生的一组霉菌毒素，使用分析级的碱钝化C8柱以及三氟乙酸缓冲液（pH 2.7）和乙腈梯度的高效液相色谱法对其进行分离。采用蒸发光散射检测器检测伏马菌素峰。具有1:14流动相分流的半制备规模碱钝化C8柱有助于伏马菌素分析标准品的纯化。