Paulus W, Baur I, Liszka U, Drlicek M, Leigh I, Bruckner-Tuderman L
Molecular Neurogenetics Unit, Massachusetts General Hospital, Charlestown, MA 02129, USA.
Virchows Arch. 1995;426(2):199-202. doi: 10.1007/BF00192642.
The distribution of type VII collagen was examined in the normal human nervous system, in brain tumour biopsies and in glioma cell lines by immunohistochemistry and western blotting. In normal tissue, positivity was observed beneath choroid plexus epithelial cells and around pineal gland and pituitary gland cell nests, while other brain regions and peripheral nerves were negative. Expression was preserved in most related tumours (choroid plexus papilloma, pineoblastoma, pituitary adenoma). Scattered abnormal vessels showed neo-expression of type VII collagen in about half of the astrocytic and ependymal tumours. Glioma cells in situ were consistently negative for type VII collagen, whereas the glioblastoma cell lines were positive. Our results suggest that anchoring fibrils or at least epitopes of their major structural component are present in normal and pathological cerebral structures, indicating a unique distribution of type VII collagen in the nervous system.
通过免疫组织化学和蛋白质印迹法，对正常人类神经系统、脑肿瘤活检组织和胶质瘤细胞系中的VII型胶原蛋白分布进行了检测。在正常组织中，脉络丛上皮细胞下方、松果体和垂体腺细胞巢周围观察到阳性反应，而其他脑区和周围神经则为阴性。在大多数相关肿瘤（脉络丛乳头状瘤、松果体母细胞瘤、垂体腺瘤）中，表达得以保留。在约一半的星形细胞和室管膜肿瘤中，散在的异常血管显示VII型胶原蛋白的新表达。原位胶质瘤细胞对VII型胶原蛋白始终呈阴性，而胶质母细胞瘤细胞系呈阳性。我们的结果表明，锚定纤维或其主要结构成分的至少表位存在于正常和病理性脑结构中，这表明VII型胶原蛋白在神经系统中具有独特的分布。
