Frandji P, Tkaczyk C, Oskéritzian C, Lapeyre J, Peronet R, David B, Guillet J G, Mécheri S
Unité d'Immuno-Allergie, Institut Pasteur, Paris, France.
Cell Immunol. 1995 Jun;163(1):37-46. doi: 10.1006/cimm.1995.1096.
We recently showed that bone marrow-derived mast cells bore MHC class II molecules and could present antigens to specific T cell hybridomas. This article summarizes the effects of purified recombinant cytokines on the expression of MHC class II molecules by mast cells and on their antigen-presenting capacity. Since IL-3 is essential for mast cell growth, all the cytokines were analyzed in the presence of IL-3. IL-3 downregulated the production of Ia molecules, so that mast cells cultured in IL-3 alone had no antigen presenting ability. In contrast, IL-4 and IFN-gamma upregulated the production of MHC class II molecules, while GM-CSF had no effect. The antigen-presenting capacity of IL-4-treated mast cells was substantially enhanced by incubating these cells with GM-CSF for 2 days. GM-CSF enhanced antigen presentation only in combination with IL-4. The activation of mast cells was reversible and could not be repeated. Finally, incubation of IL-4- or IL-4/GM-CSF-treated mast cells with IFN-gamma led to almost complete inhibition of the antigen-presenting function. These findings provide new insights into the regulation of specific allergic responses.
我们最近发现，骨髓来源的肥大细胞表达MHC II类分子，并能将抗原呈递给特定的T细胞杂交瘤。本文总结了纯化的重组细胞因子对肥大细胞MHC II类分子表达及其抗原呈递能力的影响。由于IL-3对肥大细胞生长至关重要，因此所有细胞因子均在IL-3存在的情况下进行分析。IL-3下调了Ia分子的产生，因此仅在IL-3中培养的肥大细胞没有抗原呈递能力。相反，IL-4和IFN-γ上调了MHC II类分子的产生，而GM-CSF则没有影响。将IL-4处理的肥大细胞与GM-CSF孵育2天，可显著增强其抗原呈递能力。GM-CSF仅与IL-4联合使用时才能增强抗原呈递。肥大细胞的激活是可逆的，且不能重复。最后，将IL-4或IL-4/GM-CSF处理的肥大细胞与IFN-γ孵育，几乎可完全抑制其抗原呈递功能。这些发现为特异性过敏反应的调节提供了新的见解。
