Nomura S, Kuroiwa A, Nagayama A
Anticancer and Antimicrobial Research Laboratory, Taiho Pharmaceutical Company.
Chemotherapy. 1995 Mar-Apr;41(2):77-81. doi: 10.1159/000239327.
The effects of the sub-MIC of antibiotics on the surface hydrophobicity and charge of Staphylococcus aureus were examined by the contact angle method and by microscopic electrophoresis, and the production of oxygen-derived radicals by mouse peritoneal macrophages was measured by a luminol-chemiluminescence assay. The treatment of the bacterial cells with antibiotics induced an increase in hydrophobicity and a decrease in the negative charge of the bacterial surface. The chemiluminescence of the macrophages stimulated by S. aureus treated with antibiotics was significantly higher than that obtained with the untreated bacterial cells. These findings suggest that the antibiotics caused an increase in the hydrophobicity and a decrease in the negative charge of the surface of S. aureus, resulting in the enhancement of nonopsonic phagocytosis of S. aureus by macrophages.
采用接触角法和显微电泳法研究了亚抑菌浓度抗生素对金黄色葡萄球菌表面疏水性和电荷的影响，并用鲁米诺化学发光法测定了小鼠腹腔巨噬细胞产生氧自由基的情况。用抗生素处理细菌细胞会导致疏水性增加和细菌表面负电荷减少。用抗生素处理的金黄色葡萄球菌刺激的巨噬细胞的化学发光明显高于未处理的细菌细胞。这些发现表明，抗生素导致金黄色葡萄球菌表面疏水性增加和负电荷减少，从而增强了巨噬细胞对金黄色葡萄球菌的非调理吞噬作用。
