Loveland K L, Zlatic K, Stein-Oakley A, Risbridger G, deKretser D M
Institute of Reproduction and Development, Monash Medical Centre, Clayton, Victoria, Australia.
Mol Cell Endocrinol. 1995 Feb 27;108(1-2):155-9. doi: 10.1016/0303-7207(94)03471-5.
The expression of mRNAs encoding the platelet-derived growth factor (PDGF) subunits (PDGF A and PDGF B) and the PDGF receptor subunits (PDGF-R alpha and PDGF-R beta) was studied in cells of the rat testis. Leydig cells, primary spermatocytes, round spermatids, and cytoplasts were isolated from adult animals, and enriched preparations of Sertoli cells were obtained from 20-day-old animals. RNA from these cells was examined using Northern blots. Messenger RNA encoding both the PDGF-R alpha and PDGF-R beta subunits was observed in Leydig and Sertoli cell preparations but not in any of the germ cell samples. The Leydig cells contained predominantly mRNA encoding PDGF B subunits, while the Sertoli cells contained abundant mRNAs encoding both ligand subunits. Neither PDGF subunit mRNA was detected in the germ cells. This paper has not examined the expression of protein corresponding to the mRNAs detected but the data suggest that both paracrine and autocrine roles for PDGF are likely in the testis, and that PDGF may mediate communication between the interstitial compartment and the seminiferous epithelium.
在大鼠睾丸细胞中研究了编码血小板源性生长因子（PDGF）亚基（PDGF A和PDGF B）和PDGF受体亚基（PDGF-Rα和PDGF-Rβ）的mRNA的表达。从成年动物中分离出睾丸间质细胞、初级精母细胞、圆形精子细胞和胞质体，并从20日龄动物中获得富集的支持细胞制剂。使用Northern印迹法检测这些细胞的RNA。在睾丸间质细胞和支持细胞制剂中观察到编码PDGF-Rα和PDGF-Rβ亚基的信使RNA，但在任何生殖细胞样本中均未观察到。睾丸间质细胞主要含有编码PDGF B亚基的mRNA，而支持细胞含有丰富的编码两种配体亚基的mRNA。在生殖细胞中未检测到任何一种PDGF亚基mRNA。本文未检测与所检测到的mRNA相对应的蛋白质的表达，但数据表明PDGF在睾丸中可能具有旁分泌和自分泌作用，并且PDGF可能介导间质区室和生精上皮之间的通讯。
