Hammes H P, Ali S S, Uhlmann M, Weiss A, Federlin K, Geisen K, Brownlee M
Third Medical Department, Justus-Liebig-University of Giessen, Germany.
Diabetologia. 1995 Mar;38(3):269-73. doi: 10.1007/BF00400629.
We have previously shown that long-term administration of aminoguanidine, an inhibitor of advanced glycosylation product formation, reduces the extent of experimental diabetic retinopathy in the rat by 85%. In order to determine whether the residual retinopathy that developed despite aminoguanidine was attributable to advanced glycation endproduct formation, a time-course study was performed in three different groups of male Wistar rats: non-diabetic controls (NC), streptozotocin-diabetic controls (DC) and streptozotocin-diabetic rats treated with aminoguanidine HCL, 50 mg/100 ml drinking water (D-AG). Eyes were obtained at 24, 32, 44 and 56 weeks of diabetes/treatment duration and morphologic evaluation was done on retinal digest preparations. At 56 weeks, retinal basement membrane thickness was additionally measured. After 24 weeks of diabetes, the number of acellular capillaries was significantly elevated in DC (44.6 +/- 5.7/mm2 of retinal area, NC 19.6 +/- 4.9; p < 0.001) and increased continuously over time (DC 56 weeks 87.4 +/- 15.1; p < 0.001 vs DC24 weeks). In contrast, acellular capillaries in D-AG increased over the first 24 weeks and then remained constant for the rest of the study (D-AG 24 weeks 35.7 +/- 5.18; p < 0.01 vs NC 24 weeks and NS vs DC 24 weeks; D-AG 56 weeks 42.0 +/- 6.20; p NS vs D-AG 24 weeks).(ABSTRACT TRUNCATED AT 250 WORDS)
我们之前已经表明，长期给予氨基胍（一种晚期糖基化产物形成抑制剂）可使大鼠实验性糖尿病视网膜病变的程度降低85%。为了确定尽管使用了氨基胍仍发生的残余视网膜病变是否归因于晚期糖基化终产物的形成，我们对三组不同的雄性Wistar大鼠进行了一项时间进程研究：非糖尿病对照组（NC）、链脲佐菌素诱导的糖尿病对照组（DC）以及用盐酸氨基胍（50 mg/100 ml饮用水）治疗的链脲佐菌素诱导的糖尿病大鼠（D - AG）。在糖尿病/治疗持续24、32、44和56周时获取眼睛，并对视网膜消化制剂进行形态学评估。在56周时，还额外测量了视网膜基底膜厚度。糖尿病24周后，DC组无细胞毛细血管数量显著增加（每平方毫米视网膜面积44.6±5.7个，NC组为19.6±4.9个；p<0.001），且随时间持续增加（DC组56周时为87.4±15.1个；与DC组24周相比，p<0.001）。相比之下，D - AG组无细胞毛细血管在最初24周增加，然后在研究的其余时间保持稳定（D - AG组24周时为35.7±5.18个；与NC组24周相比，p<0.01，与DC组24周相比无显著差异；D - AG组56周时为42.0±6.20个；与D - AG组24周相比无显著差异）。（摘要截断于250字）
