Belyaev A S, Hails R S, Roy P
Laboratory of Molecular Biophysics, University of Oxford, UK.
Gene. 1995 Apr 24;156(2):229-33. doi: 10.1016/0378-1119(95)00050-g.
Co-infection with several viruses is often used to achieve simultaneous expression of several proteins. From co-infections involving several viruses, the ratio of proteins synthesized in individual cells can be very variable. This is disadvantageous where proteins are needed to interact to provide a maximum yield of a complex product. Multiple-gene-expression vectors offer an alternative to co-infections. They enable reproducible ratios of products to be provided in each infected cell. Until recently, multigene-expression vectors have only been developed to make two proteins simultaneously. Here, we describe the generation of a single recombinant baculovirus synthesising up to five foreign proteins with a fixed ratio comparable to the ratio during the synthesis of native proteins.
几种病毒的共感染常用于实现几种蛋白质的同时表达。在涉及几种病毒的共感染中，单个细胞中合成的蛋白质比例可能变化很大。在需要蛋白质相互作用以提供最大产量的复杂产物的情况下，这是不利的。多基因表达载体为共感染提供了一种替代方法。它们能够在每个感染细胞中提供可重复的产物比例。直到最近，多基因表达载体仅被开发用于同时产生两种蛋白质。在这里，我们描述了一种单一重组杆状病毒的产生，该病毒可合成多达五种外源蛋白质，其固定比例与天然蛋白质合成过程中的比例相当。
