Roy P
NERC Institute of Virology and Environmental Microbiology, Oxford, U.K.
FEMS Microbiol Immunol. 1990 Nov;2(4):223-34. doi: 10.1111/j.1574-6968.1990.tb03523.x.
The productivity and flexibility of insect baculovirus expression vectors and the ability of the baculovirus genome to incorporate (and express) large amounts of foreign DNA allows this system to be used for both single and multiple gene expression. Using the system, bluetongue virus (BTV) genes have been expressed to develop diagnostic reagents and vaccines as well as to understand the basic structures of the virions. BTV which causes disease in ruminants in many parts of the world, consists of 10 double-stranded RNA segments enclosed by double capsids that are composed by 7 structural proteins. Since each protein is encoded by a single RNA species, DNA clones of all 10 RNA species were synthesized and individually expressed in baculovirus vectors at high levels. This has yielded proteins that have been shown to be excellent diagnostic and vaccine reagents. In addition, multiple expression vectors have been used to synthesize morphological structures (viral and subviral) representing BTV.
昆虫杆状病毒表达载体的生产力和灵活性，以及杆状病毒基因组整合（并表达）大量外源DNA的能力，使得该系统可用于单基因和多基因表达。利用该系统，已表达蓝舌病毒（BTV）基因以开发诊断试剂和疫苗，并了解病毒粒子的基本结构。BTV在世界许多地区的反刍动物中引发疾病，由10个双链RNA片段组成，被由7种结构蛋白构成的双层衣壳所包裹。由于每种蛋白质由单一RNA种类编码，因此合成了所有10种RNA种类的DNA克隆，并在杆状病毒载体中高水平地单独表达。这产生了已被证明是优良诊断和疫苗试剂的蛋白质。此外，已使用多个表达载体来合成代表BTV的形态结构（病毒和亚病毒）。
