Mullany P, Wilks M, Tabaqchali S
Department of Medical Microbiology, St Bartholomew's Hospital Medical College, West Smithfield, London, UK.
J Antimicrob Chemother. 1995 Feb;35(2):305-15. doi: 10.1093/jac/35.2.305.
An MLS resistance gene designated ermBZ, from a toxigenic Clostridium difficile strain (630) could be transferred between C. difficile strains, and to and from Bacillus subtilis. The intergeneric transfer occurred in the absence of any detectable plasmid DNA and the element responsible for gene transfer entered the recipient's chromosome, behaviour which is characteristic of a conjugative transposon. The element was designated Tn5398 and was found in six C. difficile strains. Tn5398 could be transferred to the non-toxigenic strain C. difficile CD37 which lacks the genes for toxins A and B. Transconjugants from both C. difficile and B. subtilis that had received the ermBZ gene also acquired a sequence of DNA that was homologous to the part of the toxin A gene that coded for the C-terminal repeat region.
从产毒艰难梭菌菌株（630）中分离出的一个名为ermBZ的大环内酯-林可酰胺-链阳霉素B（MLS）抗性基因，可以在艰难梭菌菌株之间转移，也可以在艰难梭菌与枯草芽孢杆菌之间相互转移。这种属间转移在没有任何可检测到的质粒DNA的情况下发生，负责基因转移的元件进入受体的染色体，这种行为是接合转座子的特征。该元件被命名为Tn5398，在6株艰难梭菌菌株中被发现。Tn5398可以转移到缺乏毒素A和B基因的无毒艰难梭菌菌株CD37中。接受ermBZ基因的来自艰难梭菌和枯草芽孢杆菌的接合子也获得了一段与毒素A基因中编码C端重复区域的部分同源的DNA序列。
