Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy.
J Med Microbiol. 2013 Sep;62(Pt 9):1461-1467. doi: 10.1099/jmm.0.057117-0. Epub 2013 Jun 5.
In Clostridium difficile, resistance to the macrolide-lincosamide-streptogramin B group of antibiotics generally relies on erm(B) genes. In this study, we investigated elements with a genetic organization different from Tn5398, the mobilizable non-conjugative element identified in C. difficile strain 630. Our results suggested that the elements most frequently found in strains isolated during the European surveillance study in 2005 were related to Tn6194, the conjugative transposon recently detected in different C. difficile types, including PCR-ribotype 027. We characterized a Tn6194-like and a novel element rarely found in clinical isolates. A burden on the in vitro fitness of C. difficile was observed after the acquisition of these elements as well as of Tn5398.
在艰难梭菌中，对大环内酯-林可酰胺-链阳性菌素 B 类抗生素的耐药性通常依赖 erm(B)基因。在这项研究中，我们研究了与 Tn5398 不同的遗传结构的元件，Tn5398 是在艰难梭菌 630 株中发现的可移动非共轭元件。我们的研究结果表明，在 2005 年欧洲监测研究中分离到的菌株中最常发现的元件与 Tn6194 有关，Tn6194 是最近在不同类型的艰难梭菌中发现的一种可共轭转座子，包括 PCR-核糖型 027。我们对一种 Tn6194 样的和一种在临床分离株中很少发现的新型元件进行了表征。在获得这些元件以及 Tn5398 后，观察到艰难梭菌的体外适应性负担增加。
