Bradley C R, Babb J R, Ayliffe G A
Hospital Infection Research Laboratory, City Hospital NHS Trust, Birmingham, UK.
J Hosp Infect. 1995 Feb;29(2):143-51. doi: 10.1016/0195-6701(95)90196-5.
An automated endoscope sterilizing machine, the Steris System 1 Processor, was tested for bactericidal and sporicidal efficacy. The disinfectant, peracetic acid, was diluted to 0.2% within an enclosed system. The exposure time to the disinfectant was 12 min and the overall cycle time ranged from 25-38 min, mean 29 min. Preliminary suspension tests, with and without yeast or serum, showed a log10 reduction of > 5 with Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis in 5 min with 0.2% peracetic acid. After a routine cycle in the machine, endoscopes contaminated with the same organisms showed no growth. Two of 24 spore strips, containing 10(6) B. subtilis showed a small number of survivors (less than 10 per strip). No significant damage to the endoscope was observed although the number of cycles tested was small (i.e. 31). The advantage of the system is that staff are not directly exposed to the agent, but the costs per cycle are higher than glutaraldehyde, since peracetic acid is not renewed. Unlike other automated processors the Steris machine has no cleaning cycle.
对一台自动内镜消毒机——斯泰瑞思系统1处理器进行了杀菌和杀孢子效果测试。消毒剂过氧乙酸在封闭系统中稀释至0.2%。消毒剂暴露时间为12分钟，整个循环时间为25 - 38分钟，平均29分钟。在有或没有酵母或血清的情况下进行的初步悬液试验表明，0.2%的过氧乙酸在5分钟内可使铜绿假单胞菌、金黄色葡萄球菌和枯草芽孢杆菌的数量对数减少>5。在机器中进行常规循环后，被相同微生物污染的内镜未显示有生长。24个含有10(6)枯草芽孢杆菌的芽孢条中有2个显示有少量存活菌（每条少于10个）。尽管测试的循环次数较少（即31次），但未观察到内镜有明显损坏。该系统的优点是工作人员不会直接接触到该药剂，但由于过氧乙酸不会更新，每个循环的成本高于戊二醛。与其他自动处理器不同，斯泰瑞思机器没有清洗循环。
