使用探针pMAV22对鸟分枝杆菌进行诊断性PCR扩增的特异性
Specificity of diagnostic PCR amplification for M. avium using the probe pMAV22.
作者信息
Sritharan V, Iralu J V, Barker R H
机构信息
Center for Biotechnology, Anna University, Madras, India.
出版信息
Mol Cell Probes. 1995 Feb;9(1):71-4. doi: 10.1016/s0890-8508(95)91067-0.
PMID:7760864
Abstract
We characterized the serovar specificity of the probe pMAV22 using ATCC isolates. Primers from this sequence amplified DNA from serovars 1-6, 8-10, and ATCC strain 19075 but did not amplify MAIS serovars 7, 11, 12, 14, 17-20. This confirmed that the M. avium probe pMAV22 is specific for M. avium, not M. intracellulare.
摘要
我们使用美国典型培养物保藏中心（ATCC）的菌株对探针pMAV22的血清型特异性进行了鉴定。该序列的引物能扩增血清型1 - 6、8 - 10以及ATCC菌株19075的DNA，但不能扩增MAIS血清型7、11、12、14、17 - 20的DNA。这证实了鸟分枝杆菌探针pMAV22对鸟分枝杆菌具有特异性，而非胞内分枝杆菌。
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