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鸟分枝杆菌特定DNA片段的分离及其在诊断中的可能应用。

Isolation of specific DNA fragments of Mycobacterium avium and their possible use in diagnosis.

作者信息

Thierry D, Vincent V, Clément F, Guesdon J L

机构信息

Laboratoire de Prédéveloppement des Sondes, Institut Pasteur, Paris, France.

出版信息

J Clin Microbiol. 1993 May;31(5):1048-54. doi: 10.1128/jcm.31.5.1048-1054.1993.

DOI:10.1128/jcm.31.5.1048-1054.1993
PMID:8501206
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC262878/
Abstract

We cloned and sequenced two DNA fragments (DT1 and DT6) from Mycobacterium avium serotype 2 for use in the identification of members of the M. avium-M. intracellulare complex (MAC). Reference strains of MAC belonging to serovars 1 to 28 were examined by using these DNA fragments as probes. The study revealed that the DT6 probe hybridized with DNAs from M. avium strains (serovars 1 to 6, 8 to 11, and 21), while the DT1 probe hybridized with DNAs from serovars 2, 3, 7, 12 to 20, and 23 to 25. DT1- and DT6-derived oligonucleotides were selected for use as primers in a polymerase chain reaction test. Amplification of the DT1 and DT6 sequences may provide the basis for a rapid and reliable assay for the detection of mycobacteria belonging to MAC.

摘要

我们从鸟分枝杆菌血清型2中克隆并测序了两个DNA片段（DT1和DT6），用于鉴定鸟分枝杆菌-胞内分枝杆菌复合体（MAC）的成员。使用这些DNA片段作为探针，对属于血清型1至28的MAC参考菌株进行了检测。研究表明，DT6探针与鸟分枝杆菌菌株（血清型1至6、8至11和21）的DNA杂交，而DT1探针与血清型2、3、7、12至20以及23至25的DNA杂交。选择源自DT1和DT6的寡核苷酸用作聚合酶链反应试验的引物。DT1和DT6序列的扩增可为快速可靠地检测属于MAC的分枝杆菌提供基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee2b/262878/936369c931bc/jcm00017-0049-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee2b/262878/315052579978/jcm00017-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee2b/262878/60b868ebf4ca/jcm00017-0047-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee2b/262878/875b53a87153/jcm00017-0048-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee2b/262878/936369c931bc/jcm00017-0049-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee2b/262878/315052579978/jcm00017-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee2b/262878/60b868ebf4ca/jcm00017-0047-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee2b/262878/875b53a87153/jcm00017-0048-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee2b/262878/936369c931bc/jcm00017-0049-a.jpg

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本文引用的文献

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[Taxonomic value of mycobacterial fatty acids: proposal for a method of analysis].[分枝杆菌脂肪酸的分类学价值：一种分析方法的建议]
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Serologic identification and classification of the atypical mycobacteria by their agglutination.通过凝集反应对非典型分枝杆菌进行血清学鉴定和分类。
通过多重实时聚合酶链反应快速鉴定结核分枝杆菌和非结核分枝杆菌
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A novel identification scheme for genus Mycobacterium, M. tuberculosis complex, and seven mycobacteria species of human clinical impact.一种针对分枝杆菌属、结核分枝杆菌复合群以及七种对人类临床有影响的分枝杆菌物种的新型鉴定方案。
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Identification of Mycobacterium avium genotypes with distinctive traits by combination of IS1245-based restriction fragment length polymorphism and restriction analysis of hsp65.通过基于IS1245的限制性片段长度多态性与hsp65限制性分析相结合来鉴定具有独特特征的鸟分枝杆菌基因型。
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Determination of genome size and DNA homology between an unclassified Mycobacterium species isolated from patients with Crohn's disease and other mycobacteria.从克罗恩病患者中分离出的未分类分枝杆菌与其他分枝杆菌之间基因组大小及DNA同源性的测定
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