Sivendra R
Appl Environ Microbiol. 1976 May;31(5):778-80. doi: 10.1128/aem.31.5.778-780.1976.
Atypical fermentation in the two-tube method of Hugh and Leifson (1953), whereby acid was produced in the sealed tube but not in the open tube, was determined to be the result of a preferential metabolism of peptone over the reactant carbohydrate. These atypical fermentations are only partially detected by one-tube oxidation-fermentation methods, and as such they constitute a source of limitation to the routine use of these abbreviated techniques for oxidative-fermentation determinations.
休和利夫森(1953年)双管法中的非典型发酵,即在密封管中产生酸而在开放管中不产生酸,被确定为蛋白胨比反应物碳水化合物优先代谢的结果。这些非典型发酵只能通过单管氧化发酵法部分检测到,因此它们构成了这些简化技术在常规用于氧化发酵测定时的一个限制因素。