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复制蛋白A与酵母DNA修复和DNA代谢基因中的调控元件结合。

Replication protein A binds to regulatory elements in yeast DNA repair and DNA metabolism genes.

作者信息

Singh K K, Samson L

机构信息

Department of Molecular and Cellular Toxicology, Harvard School of Public Health, Boston, MA 02115, USA.

出版信息

Proc Natl Acad Sci U S A. 1995 May 23;92(11):4907-11. doi: 10.1073/pnas.92.11.4907.

DOI:10.1073/pnas.92.11.4907
PMID:7761422
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC41816/
Abstract

Saccharomyces cerevisiae responds to DNA damage by arresting cell cycle progression (thereby preventing the replication and segregation of damaged chromosomes) and by inducing the expression of numerous genes, some of which are involved in DNA repair, DNA replication, and DNA metabolism. Induction of the S. cerevisiae 3-methyladenine DNA glycosylase repair gene (MAG) by DNA-damaging agents requires one upstream activating sequence (UAS) and two upstream repressing sequences (URS1 and URS2) in the MAG promoter. Sequences similar to the MAG URS elements are present in at least 11 other S. cerevisiae DNA repair and metabolism genes. Replication protein A (Rpa) is known as a single-stranded-DNA-binding protein that is involved in the initiation and elongation steps of DNA replication, nucleotide excision repair, and homologous recombination. We now show that the MAG URS1 and URS2 elements form similar double-stranded, sequence-specific, DNA-protein complexes and that both complexes contain Rpa. Moreover, Rpa appears to bind the MAG URS1-like elements found upstream of 11 other DNA repair and DNA metabolism genes. These results lead us to hypothesize that Rpa may be involved in the regulation of a number of DNA repair and DNA metabolism genes.

摘要

酿酒酵母通过阻止细胞周期进程（从而防止受损染色体的复制和分离）以及诱导众多基因的表达来应对DNA损伤，其中一些基因参与DNA修复、DNA复制和DNA代谢。DNA损伤剂诱导酿酒酵母3-甲基腺嘌呤DNA糖基化酶修复基因（MAG）需要MAG启动子中的一个上游激活序列（UAS）和两个上游抑制序列（URS1和URS2）。与MAG URS元件相似的序列存在于至少11个其他酿酒酵母DNA修复和代谢基因中。复制蛋白A（Rpa）是一种单链DNA结合蛋白，参与DNA复制、核苷酸切除修复和同源重组的起始和延伸步骤。我们现在表明，MAG URS1和URS2元件形成类似的双链、序列特异性DNA-蛋白质复合物，并且这两种复合物都含有Rpa。此外，Rpa似乎结合在其他11个DNA修复和DNA代谢基因上游发现的MAG URS1样元件。这些结果使我们推测Rpa可能参与许多DNA修复和DNA代谢基因的调控。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93b4/41816/a689b4045fc5/pnas01487-0199-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93b4/41816/5af41aac9836/pnas01487-0197-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93b4/41816/995770d30f2e/pnas01487-0198-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93b4/41816/f7d9ba3fc693/pnas01487-0199-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93b4/41816/a689b4045fc5/pnas01487-0199-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93b4/41816/5af41aac9836/pnas01487-0197-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93b4/41816/995770d30f2e/pnas01487-0198-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93b4/41816/f7d9ba3fc693/pnas01487-0199-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93b4/41816/a689b4045fc5/pnas01487-0199-b.jpg

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Genomic analysis of stationary-phase and exit in Saccharomyces cerevisiae: gene expression and identification of novel essential genes.酿酒酵母稳定期及退出的基因组分析：基因表达与新型必需基因的鉴定
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Plant-specific regulation of replication protein A2 (OsRPA2) from rice during the cell cycle and in response to ultraviolet light exposure.水稻中复制蛋白A2（OsRPA2）在细胞周期及紫外线照射响应过程中的植物特异性调控
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