Lidegran M, Kjörell U, Henriksson R, Forsgren S
Department of Otorhinolaryngology, Huddinge University Hospital, Karolinska Institute, Stockholm, Sweden.
Regul Pept. 1995 Feb 14;55(3):321-30. doi: 10.1016/0167-0115(94)00120-m.
It is previously well known that bombesin has effects as growth factor and that changes in bombesin content in air-way structures occur in various patho-physiological conditions. In the present study, the effects of radiotherapy on bombesin expression in the rat larynx were studied. Irradiation was given for five days, 6 or 8 gray daily. Ten days after cessation of irradiation, the subglottic part of the larynx from irradiated and control animals was dissected out, and processed for immunohistochemistry or radioimmunoassay. The radioimmunoassay analysis showed that the content of bombesin-like material increased 2-fold after irradiation. The immunohistochemical analysis displayed an increased bombesin-like immunoreactivity in local ganglionic cells and in nerve fibers in the submucosal glands. These nerve fibers are likely to be derived from the local ganglionic cells. On the other hand, there was no change in the pattern of immuno-reactivity in the innervation of the epithelium and the lamina propria, including the blood vessels. The observations show that radiotherapy can be added to the list of factors that influence bombesin expression in airway structures.
此前众所周知，蛙皮素具有生长因子的作用，且气道结构中蛙皮素含量的变化会在各种病理生理条件下发生。在本研究中，研究了放疗对大鼠喉部蛙皮素表达的影响。连续五天进行照射，每天照射6或8格雷。照射停止十天后，将照射组和对照组动物喉部的声门下部分取出，进行免疫组织化学或放射免疫测定处理。放射免疫分析表明，照射后蛙皮素样物质的含量增加了两倍。免疫组织化学分析显示，局部神经节细胞和黏膜下腺神经纤维中的蛙皮素样免疫反应性增强。这些神经纤维可能来源于局部神经节细胞。另一方面，在上皮和固有层（包括血管）的神经支配中，免疫反应模式没有变化。这些观察结果表明，放疗可被列入影响气道结构中蛙皮素表达的因素清单。
