Iushkova L F, Nekhanevich N F, Potapov V A, Romashchenko A G
Biokhimiia. 1976 Mar;41(3):420-5.
Described earlier method of isolation of E. coli DNA-polymerase is modified. In order to decrease the possible effect of proteases on DNA-polymerase, the time of autolysis was shortened. An additional stage of precipitation by ammonium sulfate of 65-80% of saturation was introduced. Chromatography on DEAE-Sephadex A-25 revealed 4 fractions exhibiting DNA-polymerase activity. It is established that combination of certain DNA-polymerase fractions, belonging to different peaks, significantly intensifies the enzyme activity. It may be due to the formation of enzymic complexes.
之前描述的大肠杆菌DNA聚合酶分离方法有所改进。为了降低蛋白酶对DNA聚合酶可能产生的影响，自溶时间被缩短。引入了一个饱和度为65 - 80%的硫酸铵额外沉淀阶段。在DEAE - 葡聚糖A - 25上进行色谱分析显示有4个具有DNA聚合酶活性的组分。已确定属于不同峰的某些DNA聚合酶组分的组合显著增强了酶活性。这可能是由于酶复合物的形成。
