类风湿性关节中的CD4 T细胞被寡克隆激活，并在疾病过程中发生变化。
CD4 T cells in the rheumatoid joint are oligoclonally activated and change during the course of disease.
作者信息
Khazaei H A, Lunardi C, So A K
机构信息
Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, United Kingdom.
出版信息
Ann Rheum Dis. 1995 Apr;54(4):314-7. doi: 10.1136/ard.54.4.314.
OBJECTIVE
To assess the nature of T cell receptor (TCR) utilisation by CD4 T cells in the rheumatoid joint.
METHODS
Sequencing of the joining (NDJ) region of TCR beta chain mRNA isolated from synovial fluid CD4 T cells was performed in three patients in order to determine if oligoclonal expansion of particular sequences was present. Two patients were studied longitudinally to determine if these sequences changed over time.
RESULTS
A number of dominant clonotypes were found within the TCR transcripts sequenced in each patient. In the two patients who were studied longitudinally, different dominant clonotypes were detected over time. No single clonotype was persistently dominant during the period of study.
CONCLUSIONS
The pattern of TCR usage showed multiple oligoclonally expanded CD4 T cells within the rheumatoid joint. The change in clonotypes within the joint over time suggests that different antigens may be able to elicit synovial inflammation during the course of rheumatoid disease.
目的
评估类风湿关节中CD4 T细胞T细胞受体（TCR）的利用情况。
方法
对从三名患者滑液CD4 T细胞中分离出的TCRβ链mRNA的连接（NDJ）区域进行测序，以确定是否存在特定序列的寡克隆扩增。对两名患者进行纵向研究，以确定这些序列是否随时间变化。
结果
在每个患者测序的TCR转录本中发现了许多优势克隆型。在纵向研究的两名患者中，随时间检测到不同的优势克隆型。在研究期间没有单一克隆型持续占主导地位。
结论
TCR使用模式显示类风湿关节内有多个寡克隆扩增的CD4 T细胞。关节内克隆型随时间的变化表明，在类风湿疾病过程中不同抗原可能引发滑膜炎症。
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