Hanses A, Spahn-Langguth H, Mutschler E
Pharmakologisches Institut für Naturwissenschaftler, Johann Wolfgang Goethe-Universität, Frankfurt/M., Germany.
Arch Pharm (Weinheim). 1995 Mar;328(3):257-60. doi: 10.1002/ardp.19953280309.
A new rapid and sensitive reversed-phase HPLC method for the routine analysis of diclofenac in human plasma is described. The method shows high selectivity, sensitivity, and reproducibility employing 4'-methoxydiclofenac as internal standard (IS) with UV-detection at 282 nm. The correlation of peak area ratios (diclofenac/IS) versus diclofenac plasma concentrations are linear between 5.0-2000 ng/ml with a detection limit of 3 ng/ml. Intra- and interday precision studies yielded coefficients of variation < 6.2% for all concentrations (range 20.0-1600 ng/ml). The method was applied for the assay of diclofenac in plasma of healthy volunteers after p.o. and rectal dosage of 50 mg diclofenac sodium and was found to be highly suitable for routine analyses.
本文描述了一种用于常规分析人血浆中双氯芬酸的快速灵敏的反相高效液相色谱新方法。该方法以4'-甲氧基双氯芬酸为内标（IS），在282nm处进行紫外检测，具有高选择性、灵敏度和重现性。峰面积比（双氯芬酸/内标）与双氯芬酸血浆浓度之间的相关性在5.0 - 2000 ng/ml范围内呈线性，检测限为3 ng/ml。日内和日间精密度研究表明，所有浓度（范围为20.0 - 1600 ng/ml）的变异系数均< 6.2%。该方法应用于50mg双氯芬酸钠口服和直肠给药后健康志愿者血浆中双氯芬酸的测定，结果表明该方法非常适合常规分析。
