Miller R B
Analytical Research Center, L.A.B. Bio-Research International Inc., Senneville, Montreal, Quebec, Canada.
J Chromatogr. 1993 Jul 2;616(2):283-90. doi: 10.1016/0378-4347(93)80396-l.
A validated high-performance liquid chromatographic procedure employing ultraviolet detection for the analysis of diclofenac in human plasma is reported. The method is rapid and, coupled with column switching, leads to a sensitive, accurate and reproducible assay. The retention times of diclofenac and the internal standard (4'-methoxydiclofenac, CGP-4287) are 6.4 and 7.6 min, respectively. The peak height versus plasma concentration is linear over the range 5.0-2000 ng/ml with a detection limit below 2.5 ng/ml. The mean absolute recovery of diclofenac using the described assay is 96.5% (n = 24). The inter- and intra-day accuracy and precision are within 8.3% of the actual values for all concentrations investigated. Furthermore, this procedure is applied to assess the pharmacokinetics of a single 75-mg oral dose of diclofenac sodium.
报道了一种经过验证的高效液相色谱法，该方法采用紫外检测来分析人血浆中的双氯芬酸。该方法快速，结合柱切换技术，可实现灵敏、准确且可重复的测定。双氯芬酸和内标物（4'-甲氧基双氯芬酸，CGP-4287）的保留时间分别为6.4分钟和7.6分钟。峰高与血浆浓度在5.0 - 2000 ng/ml范围内呈线性关系，检测限低于2.5 ng/ml。使用所述测定法时，双氯芬酸的平均绝对回收率为96.5%（n = 24）。对于所有研究浓度，日内和日间的准确度和精密度均在实际值的8.3%以内。此外，该方法用于评估单次口服75 mg双氯芬酸钠的药代动力学。
