用于测定人血浆和尿液中双氯芬酸的无萃取高效液相色谱法。

Extractionless high-performance liquid chromatographic method for the determination of diclofenac in human plasma and urine.

作者信息

Avgerinos A, Karidas T, Malamataris S

机构信息

Laboratory of Pharmaceutical Technology, University of Thessaloniki, Greece.

出版信息

J Chromatogr. 1993 Sep 22;619(2):324-9. doi: 10.1016/0378-4347(93)80125-n.

DOI:10.1016/0378-4347(93)80125-n

PMID:8263107

Abstract

An assay using reversed-phase high-performance liquid chromatography with ultraviolet detection, at 278 nm, was developed to measure diclofenac in human plasma and urine at concentrations suitable for biopharmaceutical studies. Indomethacin was used as internal standard and separation was performed at 40 degrees C on a C18 Spherisorb column with acetonitrile-0.1 M sodium acetate (35:65, v/v) (pH 6.3) as mobile phase. The sample preparation is simple and rapid (extractionless), and the total run time is less than 5 min. The retention time is 2.8 min for diclofenac and 3.6 min for indomethacin. The detection limit is 0.2 microgram/ml using a 20-microliters loop.

摘要

开发了一种使用反相高效液相色谱法并结合278 nm紫外检测的分析方法，用于测定人血浆和尿液中双氯芬酸的浓度，该浓度适用于生物制药研究。吲哚美辛用作内标，在40℃下于C18 Spherisorb柱上进行分离，以乙腈-0.1 M醋酸钠（35:65，v/v）（pH 6.3）作为流动相。样品制备简单快速（无需萃取），总运行时间少于5分钟。双氯芬酸的保留时间为2.8分钟，吲哚美辛的保留时间为3.6分钟。使用20微升定量环时，检测限为0.2微克/毫升。