过氧亚硝酸盐会导致质粒pBR322中的DNA切口。
Peroxynitrite causes DNA nicks in plasmid pBR322.
作者信息
Salgo M G, Stone K, Squadrito G L, Battista J R, Pryor W A
机构信息
Biodynamics Institute, Louisiana State University, Baton Rouge 70803-1800, USA.
出版信息
Biochem Biophys Res Commun. 1995 May 25;210(3):1025-30. doi: 10.1006/bbrc.1995.1759.
Peroxynitrite causes single-strand breaks in pBR322 supercoiled DNA as evidenced by agarose gel electrophoresis analysis. The effect of three free radical scavengers, namely mannitol, benzoate and dimethylsulfoxide, were studied. Mannitol failed to protect DNA from damage by peroxynitrite while benzoate and dimethylsulfoxide amplified the damage. These results suggest the damage caused by peroxynitrite alone is not mediated by free radicals since typical free radical scavengers fail to prevent the damage.
过氧亚硝酸盐会导致pBR322超螺旋DNA出现单链断裂，这一点通过琼脂糖凝胶电泳分析得到了证实。研究了三种自由基清除剂，即甘露醇、苯甲酸盐和二甲基亚砜的作用。甘露醇无法保护DNA免受过氧亚硝酸盐的损伤，而苯甲酸盐和二甲基亚砜则加剧了这种损伤。这些结果表明，仅由过氧亚硝酸盐造成的损伤并非由自由基介导，因为典型的自由基清除剂无法防止这种损伤。
Zotero 插件