Villechanoux S, Garnier M, Laigret F, Renaudin J, Bové J M
Laboratory of Cellular and Molecular Biology, National Institute of Agronomic Research, Villenave d'Ornon, France.
Curr Microbiol. 1993 Mar;26(3):161-6. doi: 10.1007/BF01577372.
We have recently cloned three DNA fragments (In-2.6, In-1.0, and In-0.6) of the non-cultured, bacterial-like organism (BLO) associated with citrus greening disease. Nucleotide sequence determination has shown that fragment In-2.6 is part of the rplKAJL-rpoBC gene cluster, a well-known operon in eubacteria. The DNA fragment upstream of and partially overlapping with In-2.6 could be isolated and was shown to be the nusG gene. In Escherichia coli, nusG is also immediately upstream of rplKAJL-rpoBC. Fragment In-1.0 carries the gene for a bacteriophage type DNA polymerase. Fragment In-0.6 could not be identified. When In-2.6 was used, at high stringency, as a probe to detect greening BLO strains in infected plants, hybridization was obtained with all Asian strains tested, but not with the African strain examined. At lower stringencies, In-2.6 was able to detect also the African strain. The implications of these results in the taxonomical position of the greening BLO are discussed.
我们最近克隆了与柑橘黄龙病相关的未培养类细菌生物（BLO）的三个DNA片段（In-2.6、In-1.0和In-0.6）。核苷酸序列测定表明，片段In-2.6是rplKAJL-rpoBC基因簇的一部分，这是真细菌中一个著名的操纵子。可以分离出In-2.6上游且部分与之重叠的DNA片段，结果表明它是nusG基因。在大肠杆菌中，nusG也紧邻rplKAJL-rpoBC的上游。片段In-1.0携带一种噬菌体类型DNA聚合酶的基因。片段In-0.6无法识别。当使用In-2.6在高严谨度下作为探针检测受感染植物中的黄龙病BLO菌株时，与所有测试的亚洲菌株都获得了杂交信号，但与检测的非洲菌株没有杂交信号。在较低严谨度下，In-2.6也能够检测到非洲菌株。讨论了这些结果对黄龙病BLO分类地位的影响。
