Ludwig A, Kretzmer G
Institut für Technische Chemie, Universität Hannover, Germany.
J Biotechnol. 1993 Jan;27(2):217-23. doi: 10.1016/0168-1656(93)90110-9.
Anchorage-dependent recombinant BHK-21 cells, which produce beta-D-galactosidase, were subjected to shear stress levels in the range between 0.1 and 1.55 N m-2 over a period of three days. To investigate cell constitution and productivity, the cell number and viability, as well as the lactate dehydrogenase and beta-D-galactosidase activity, were determined. Low (0.25-0.75 N m-2) and intermediate (0.75-1.25 N m-2) shear stress rates stimulated; higher shear stress rates (more than 1.25 N m-2) diminished productivity. Cell number and general condition were influenced negatively by all but the lowest levels of shear stress. An optimal shear stress level between 0.4 and 0.6 N m-2 can be deduced.
产生β-D-半乳糖苷酶的贴壁依赖性重组BHK-21细胞在三天时间内承受了0.1至1.55 N m-2范围内的剪切应力水平。为了研究细胞组成和生产力，测定了细胞数量和活力，以及乳酸脱氢酶和β-D-半乳糖苷酶活性。低（0.25-0.75 N m-2）和中等（0.75-1.25 N m-2）剪切应力速率有刺激作用；较高的剪切应力速率（超过1.25 N m-2）会降低生产力。除了最低水平的剪切应力外，所有剪切应力水平都会对细胞数量和总体状况产生负面影响。可以推断出最佳剪切应力水平在0.4至0.6 N m-2之间。
