添加胆固醇对贴壁哺乳动物细胞生长动力学和剪切应力敏感性的影响。

Effect of cholesterol addition on growth kinetics and shear stress sensitivity of adherent mammalian cells.

作者信息

Tomeczkowski J, Ludwig A, Kretzmer G

机构信息

Medizinische Hochschule Hannover, Germany.

出版信息

Enzyme Microb Technol. 1993 Oct;15(10):849-53. doi: 10.1016/0141-0229(93)90096-k.

DOI:10.1016/0141-0229(93)90096-k

PMID:7764103

Abstract

The growth kinetics of adherent baby hamster kidney cells cultivated with additional cholesterol as well as the effect of cholesterol addition on shear stress sensitivity were investigated. The influence of various cholesterol preparations was tested, whereby dimethylsulfoxide and ethanol show negative effects at higher concentrations. With addition of cholesterol in the range of 90 micrograms ml-1, a positive effect on the shear stress resistance was achieved.

摘要

研究了添加胆固醇培养的贴壁幼仓鼠肾细胞的生长动力学，以及添加胆固醇对剪切应力敏感性的影响。测试了各种胆固醇制剂的影响，结果表明，二甲基亚砜和乙醇在较高浓度下具有负面影响。当添加90微克/毫升范围内的胆固醇时，可提高对剪切应力的耐受性。