Ludwig A, Kretzmer G, Schügerl K
Institut für Technische Chemie, Universität Hannover, FRG.
Enzyme Microb Technol. 1992 Mar;14(3):209-13. doi: 10.1016/0141-0229(92)90068-y.
An apparatus for the detailed investigation of the influence of shear stress on adherent BHK cells was developed. Shear forces between 0.0 and 2.5 N m-2 were studied. The influence on cell viability, cell morphology, cell lysis, and cell size was determined. Increasing shear forces as well as increasing exposure duration caused increasing changes in cell morphology and cell death. A "critical shear stress level" was determined.
开发了一种用于详细研究剪切应力对贴壁BHK细胞影响的装置。研究了0.0至2.5 N m-2之间的剪切力。确定了对细胞活力、细胞形态、细胞裂解和细胞大小的影响。剪切力增加以及暴露时间延长导致细胞形态和细胞死亡的变化增加。确定了一个“临界剪切应力水平”。
