Koga T, Kikuchi M
Research and Development Division, Kikkoman Corporation, Chiba, Japan.
Biosci Biotechnol Biochem. 1993 Mar;57(3):367-71. doi: 10.1271/bbb.57.367.
A novel immunomodulating fraction, SP-MAF1, was isolated from soybeans as a polysaccharide-protein complex by hot water extraction followed by acid treatment, ethanol treatment, and chromatography on a DEAE-Sepharose CL-6B column, and was characterized in some biological activities. SP-MAF1 increased glycolysis and IL-1 production by macrophages, but did not affect TNF production. SP-MAF1 also increased splenocyte proliferation that was induced by LPS and ConA, and ConA-induced production of IL-2 by splenocytes. In an in vivo test, the mean survival times of SP-MAF1-treated mice bearing FM3A were significantly longer than those of non-treated group. Since SP-MAF1 affected macrophages and splenocytes but not tumor cell growth, this effect may be mediated by the host immune system. Furthermore SP-MAF1 had a capacity to suppress the TNBS-induced DTH reaction. These results suggested that SP-MAF1 could either enhance or suppress immune functions.
一种新型免疫调节组分SP-MAF1，通过热水提取，随后进行酸处理、乙醇处理，并在DEAE-琼脂糖CL-6B柱上进行层析，从大豆中分离得到，为一种多糖-蛋白质复合物，并对其一些生物学活性进行了表征。SP-MAF1可增加巨噬细胞的糖酵解和白细胞介素-1的产生，但不影响肿瘤坏死因子的产生。SP-MAF1还可增加脂多糖（LPS）和刀豆蛋白A（ConA）诱导的脾细胞增殖，以及ConA诱导的脾细胞白细胞介素-2的产生。在体内试验中，经SP-MAF1处理的携带FM3A肿瘤的小鼠的平均存活时间显著长于未处理组。由于SP-MAF1影响巨噬细胞和脾细胞，但不影响肿瘤细胞生长，这种作用可能是由宿主免疫系统介导的。此外，SP-MAF1具有抑制三硝基苯磺酸（TNBS）诱导的迟发型超敏反应（DTH）的能力。这些结果表明，SP-MAF1既可以增强也可以抑制免疫功能。
