Lidén G, Niklasson C
Department of Chemical Reaction Engineering, Chalmers University of Technology, Göteborg, Sweden.
Biotechnol Prog. 1993 Mar-Apr;9(2):179-85. doi: 10.1021/bp00020a010.
Measurements on pure solutions of NADH (8-800 microM) were made at different path lengths (0-140 mm) with an open-ended fluorosensor. It was shown that a geometric factor must be included in a model to quantitatively explain the results. A mathematical expression for this factor was found, and parameter estimations were made. It was shown that the often-used implicit assumption of a completely directed emitted light is not correct. A better fit was obtained by assuming the emitted light to be uniformly distributed in all directions. However, it was necessary to consider the slight divergence of the excitation light beams in the model.
使用开放式荧光传感器，在不同光程长度（0 - 140毫米）下对NADH纯溶液（8 - 800微摩尔）进行了测量。结果表明，在模型中必须包含一个几何因子，以便定量解释测量结果。找到了该因子的数学表达式，并进行了参数估计。结果表明，通常使用的完全定向发射光的隐含假设是不正确的。通过假设发射光在所有方向上均匀分布，可以得到更好的拟合。然而，在模型中必须考虑激发光束的轻微发散。
