Cabané M, Calvet P, Vincens P, Boudet A M
Centre de Biologie et Physiologie Végétales, URA CNRS 1457, Toulouse, France.
Planta. 1993;190(3):346-53. doi: 10.1007/BF00196963.
Through a 5-d exposure at 14 degrees C/8 degrees C (day/night), soybean (Glycine max [L.] Merr.) was acclimated to a lower temperature of 8 degrees C. In order to assess changes in protein synthesis related to chilling acclimation, proteins were labeled in vivo with [35S]methionine, separated by two-dimensional gel electrophoresis, and the derived autoradiograms were subjected to computer analysis. Two sets of chilling-acclimation-related proteins were characterized following exposure and labeling at 8 degrees C. One set corresponded to proteins whose synthesis was stimulated in acclimated plants in comparison with non-acclimated plants after transfer to 8 degrees C for 2 d. The other set also displayed an enhanced synthesis in the acclimated plants versus the non-acclimated plants but after 7 d of exposure at 8 degrees C. Most of these chilling-acclimation-related proteins were not increased during the acclimation period at 14 degrees C. Using microsequence analysis, one of these proteins was shown to have a high sequence homology with members of the heat-shock protein (HSP 70) family.
通过在14℃/8℃（白天/夜晚）下进行5天的暴露处理，大豆（Glycine max [L.] Merr.）适应了8℃的低温。为了评估与冷驯化相关的蛋白质合成变化，用[35S]甲硫氨酸在体内对蛋白质进行标记，通过二维凝胶电泳进行分离，并对所得放射自显影片进行计算机分析。在8℃下暴露和标记后，鉴定出两组与冷驯化相关的蛋白质。一组对应于在转移到8℃ 2天后，与未驯化植物相比，在驯化植物中合成受到刺激的蛋白质。另一组在驯化植物中与未驯化植物相比，在8℃暴露7天后也显示出合成增强。这些与冷驯化相关的蛋白质大多数在14℃的驯化期内并未增加。通过微序列分析，其中一种蛋白质显示与热休克蛋白（HSP 70）家族成员具有高度序列同源性。
