Manganaro J L, Goldberg B S
FMC Corporation, Princeton, New Jersey 08543.
Biotechnol Prog. 1993 May-Jun;9(3):285-90. doi: 10.1021/bp00021a007.
Novel porous sheets containing commercially available cellulosic ion exchange media of several different functionalities in a PVC matrix have been prepared and evaluated. The advantages of these materials are resistance to alkali, clean in place procedures and low nonspecific binding. These sheets thus provide well-known and well-characterized particulate chromatographic media in a porous sheet format. The porous sheet format permits use of short, squat geometries (stacked sheets) as well as tolerance of high pressure gradients. The net result is the ability to handle much higher flow rates (10-fold or greater) than achievable by comparable cellulosic packed columns of the neat particulate media. Chromatographic separation of natural egg white proteins by a linear salt gradient on a single sheet of 1.3-mm thickness was demonstrated. In addition, a very significant advantage of the porous sheet format is that it permits the fabrication of devices which are not possible with particulate media. An example of such a device is the Acti-Mod Spiral Module. In the spiral module a spiral flow channel is formed by wrapping the porous sheet material around a mandrel. Embossed ribbing in the porous sheet provides channel spacing. The small open channel accommodates high flow rates of biological suspensions while the porous sheet walls selectively remove components of the suspension.
已制备并评估了新型多孔片材，其在PVC基质中包含几种具有不同功能的市售纤维素离子交换介质。这些材料的优点是耐碱、原位清洁程序以及低非特异性结合。因此，这些片材以多孔片材形式提供了众所周知且特性良好的颗粒色谱介质。多孔片材形式允许使用短而矮的几何形状（堆叠片材）以及耐受高压梯度。最终结果是能够处理比可比的纯颗粒介质纤维素填充柱所能达到的流速高得多（10倍或更高）的流速。通过在一片1.3毫米厚的片材上进行线性盐梯度对天然蛋清蛋白进行了色谱分离。此外，多孔片材形式的一个非常显著的优点是它允许制造颗粒介质无法制造的装置。这种装置的一个例子是Acti-Mod螺旋模块。在螺旋模块中，通过将多孔片材材料缠绕在心轴上形成螺旋流动通道。多孔片中的压纹肋提供通道间距。小的开放通道可容纳高流速的生物悬浮液，而多孔片材壁可选择性地去除悬浮液的成分。
