Department of Parasitic Disease Research, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
Veterinary Medicine Department, Isfahan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), P.O. Box: 81785-199, Amirieh town, Isfahan, Iran.
Parasitol Res. 2021 May;120(5):1915-1919. doi: 10.1007/s00436-021-07119-6. Epub 2021 Mar 19.
This study reports the identification and first molecular characterization of Babesia occultans from naturally infected cows in Iran. Microscopic examination showed pyriform trophozoites, and ring-shaped merozoites (>2.5 μm) in Giemsa-stained blood smears obtained from two symptomatic cows in West-Azarbaijan province, Iran. PCR amplification of the partial 18S rRNA gene including the V4 hypervariable region were carried out on DNA extracted from blood samples. BLAST analyses of the partial 18S rRNA (approximately 400 bp) obtained from two cows revealed the presence of B. occultans and the detected sequences were identical to each other. Comparisons of the partial 18S rRNA sequence of the current isolate with other B. occultans sequences from Tunisia, South Africa, Turkey, Pakistan, and China confirmed the relation of the Iranian isolate to the species B. occultans. Sequence analysis of the obtained B. occultans showed 99.5-100% identity to the previously reported isolates. The sequences of B. occultans had 100% identity to a sequence obtained from ticks in Tunisia. This report is the beginning of a path to further research about B. occultans in vectors and reservoirs throughout Iran.
本研究报告了在伊朗自然感染牛中发现并首次对巴贝西虫属隐匿种进行的分子鉴定。从伊朗东阿塞拜疆省的两只症状明显的牛的吉氏染色血液涂片观察到，呈梨形的滋养体和呈环形的裂殖体（>2.5 μm）。对从血液样本中提取的 DNA 进行的部分 18S rRNA 基因（包括 V4 高变区）PCR 扩增，对来自两只牛的部分 18S rRNA（约 400 bp）进行的 BLAST 分析显示存在巴贝西虫属隐匿种，并且检测到的序列彼此相同。将当前分离株的部分 18S rRNA 序列与来自突尼斯、南非、土耳其、巴基斯坦和中国的其他巴贝西虫属隐匿种序列进行比较，证实了伊朗分离株与物种巴贝西虫属隐匿种的关系。获得的巴贝西虫属隐匿种序列分析显示与先前报道的分离株具有 99.5-100%的同一性。巴贝西虫属隐匿种的序列与从突尼斯的蜱中获得的序列完全相同。本报告是对伊朗整个地区的巴贝西虫属隐匿种在媒介和宿主中进一步研究的开始。
