Hatta H, Tsuda K, Akachi S, Kim M, Yamamoto T, Ebina T
Central Research Laboratories, Taiyo Kagaku Co., Ltd., Mie, Japan.
Biosci Biotechnol Biochem. 1993 Jul;57(7):1077-81. doi: 10.1271/bbb.57.1077.
The neutralization titer of anti-human rotavirus (HRV) IgY was completely inactivated by pepsin at pH 2.0. However, it was not significantly affected by trypsin or chymotrypsin under certain conditions. The immunological activity of the IgY was observed in the intestine of suckling mice for 2 h after oral administration and the activity rapidly decreased thereafter. The effects of oral supply of IgY were thus estimated for HRV-induced diarrhea in suckling mice and it was found that a previous supply of the IgY (1 h before HRV infection) completely prevented the HRV-induced diarrhea. The preventive effect was decreased as the time gap between IgY administration and HRV infection was longer. However, the oral supply of the IgY within 24 h after HRV infection was still effective and decreased the incidence of HRV diarrhea in suckling mice.
抗人轮状病毒（HRV）IgY的中和效价在pH 2.0时被胃蛋白酶完全灭活。然而，在某些条件下，它不受胰蛋白酶或糜蛋白酶的显著影响。口服IgY后，在乳鼠肠道中观察到其免疫活性持续2小时，此后活性迅速下降。因此评估了口服IgY对乳鼠HRV诱导腹泻的影响，发现预先提供IgY（HRV感染前1小时）可完全预防HRV诱导的腹泻。随着IgY给药与HRV感染之间的时间间隔延长，预防效果降低。然而，HRV感染后24小时内口服IgY仍然有效，并降低了乳鼠HRV腹泻的发生率。
