Clarkson A I, Lefevre P, Titchener-Hooker N J
Department of Chemical and Biochemical Engineering, University College London, U.K.
Biotechnol Prog. 1993 Sep-Oct;9(5):462-7. doi: 10.1021/bp00023a004.
The effects of varying the levels of cell disruption achieved by high-pressure homogenization upon subsequent centrifugal separation of cell debris have been defined by measuring sedimentation velocity distributions of the cell debris. Using this data together with particle size distributions, a computer-based model of a disk stack centrifuge has been used to predict the efficiency of centrifugal separation. These predictions have been compared with both laboratory scale and pilot plant scale experimental results. A similar sedimentation velocity technique has been used to characterize the properties of the flocs formed by addition of poly(ethylene imine) to a borax-clarified homogenate supernatant. The variations of floc settling properties with respect to polymer concentration are defined.
通过测量细胞碎片的沉降速度分布，确定了高压匀浆实现的不同细胞破碎水平对后续细胞碎片离心分离的影响。利用这些数据以及粒度分布，基于计算机的碟片式离心机模型已被用于预测离心分离效率。这些预测结果已与实验室规模和中试工厂规模的实验结果进行了比较。一种类似的沉降速度技术已被用于表征通过向硼砂澄清的匀浆上清液中添加聚乙烯亚胺形成的絮凝物的特性。确定了絮凝物沉降特性随聚合物浓度的变化情况。
