硅酮消泡剂对鼓泡培养中杂交瘤细胞剪切敏感性的影响。
Effect of silicone antifoam on shear sensitivity of hybridoma cells in sparged cultures.
作者信息
van der Pol L A, Bonarius D, van de Wouw G, Tramper J
机构信息
Bio-Intermediair, Groningen, The Netherlands.
出版信息
Biotechnol Prog. 1993 Sep-Oct;9(5):504-9. doi: 10.1021/bp00023a009.
PMID:7764164
Abstract
Addition of high concentrations of silicone antifoam (AF) to a suspension of hybridoma cells in a bubble column reduces the death rate when using medium without a protective component. In contrast, high AF concentrations can cause detrimental effects in a sparged medium with a protective component, such as is applied for large-scale animal cell cultures.
摘要
在气泡柱中向杂交瘤细胞悬液添加高浓度的有机硅消泡剂（AF），在使用不含保护成分的培养基时可降低死亡率。相比之下，高浓度的AF在含有保护成分的鼓泡培养基中可能会产生有害影响，例如在大规模动物细胞培养中所使用的培养基。
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