McElroy D, Rothenberg M, Reece K S, Wu R
Field of Botany, Cornell University, Ithaca, New York 14853.
Plant Mol Biol. 1990 Aug;15(2):257-68. doi: 10.1007/BF00036912.
Four rice (Oryza sativa) genomic actin genes have been characterized. The rice actin genes show a conservation of intron number and position that is characteristic of plant actins. Sequence comparisons revealed that the plant actins generally have a high degree of inter- and intraspecific sequence heterogeneity. However, one rice actin gene has a much higher degree of nucleotide sequence identity to a previously isolated actin sequence from Arabidopsis thaliana than to any other plant actin gene. This leads us to suggest that the two sequences may represent functionally homologous genes which arose from an ancient actin gene lineage that was separated by the divergence of the dicot and monocot plants. Genomic DNA blot analysis showed that the rice actin gene family contains at least eight unique members. RNA hybridization analysis revealed that individual rice actin genes can display different patterns of transcript accumulation. The observed differences in sequence and transcript accumulation patterns suggest that the individual rice actin genes may differ in their transcriptional regulation and/or cellular function.
已对四个水稻（Oryza sativa）基因组肌动蛋白基因进行了表征。水稻肌动蛋白基因显示出内含子数量和位置的保守性，这是植物肌动蛋白的特征。序列比较表明，植物肌动蛋白通常在种间和种内具有高度的序列异质性。然而，一个水稻肌动蛋白基因与先前从拟南芥中分离出的肌动蛋白序列的核苷酸序列同一性，比与任何其他植物肌动蛋白基因的都要高得多。这使我们推测，这两个序列可能代表功能同源的基因，它们起源于一个古老的肌动蛋白基因谱系，该谱系因双子叶植物和单子叶植物的分化而分离。基因组DNA印迹分析表明，水稻肌动蛋白基因家族至少包含八个独特成员。RNA杂交分析显示，单个水稻肌动蛋白基因可表现出不同的转录本积累模式。观察到的序列和转录本积累模式的差异表明，单个水稻肌动蛋白基因在转录调控和/或细胞功能上可能存在差异。
