Dequin S, Barre P
Laboratoire de Microbiologie et Technologie des Fermentations, Institut des Produits de la Vigne, INRA, Montpellier, France.
Biotechnology (N Y). 1994 Feb;12(2):173-7. doi: 10.1038/nbt0294-173.
We describe the construction of a Saccharomyces cerevisiae strain expressing the gene encoding the L(+)-lactate dehydrogenase [L(+)-LDH)] from Lactobacillus casei. The recombinant strain is able to perform a mixed lactic acid-alcoholic fermentation. Yeast cells expressing the L(+)-LDH gene from the yeast alcohol dehydrogenase (ADH1) promoter on a multicopy plasmid simultaneously convert glucose to both ethanol and lactate, with up to 20% of the glucose transformed into L(+)-lactate. Such strains may be used in every field where both biological acidification and alcoholic fermentation are required.
我们描述了一种酿酒酵母菌株的构建，该菌株表达来自干酪乳杆菌的编码L(+)-乳酸脱氢酶[L(+)-LDH]的基因。该重组菌株能够进行乳酸-酒精混合发酵。在多拷贝质粒上从酵母乙醇脱氢酶（ADH1）启动子表达L(+)-LDH基因的酵母细胞能同时将葡萄糖转化为乙醇和乳酸，高达20%的葡萄糖被转化为L(+)-乳酸。此类菌株可用于需要生物酸化和酒精发酵的各个领域。
