Brambilla L, Bolzani D, Compagno C, Carrera V, van Dijken J P, Pronk J T, Ranzi B M, Alberghina L, Porro D
Dipartimento di Fisiologia e Biochimica Generali, Università degli Studi di Milano, Italy.
FEMS Microbiol Lett. 1999 Feb 15;171(2):133-40. doi: 10.1111/j.1574-6968.1999.tb13423.x.
Introduction of the Lactobacillus casei lactate dehydrogenase (LDH) gene into Saccharomyces cerevisiae under the control of the TPI1 promoter yielded high LDH levels in batch and chemostat cultures. LDH expression did not affect the dilution rate above which respiro-fermentative metabolism occurred (Dc) in aerobic, glucose-limited chemostats. Above Dc, the LDH-expressing strain produced both ethanol and lactate, but its overall fermentation rate was the same as in wild-type cultures. Exposure of respiring, LDH-expressing cultures to glucose excess triggered simultaneous ethanol and lactate production. However, the specific glucose consumption rate was not affected, indicating that NADH reoxidation does not control glycolytic flux under these conditions.
在TPI1启动子的控制下，将干酪乳杆菌乳酸脱氢酶（LDH）基因导入酿酒酵母，在分批培养和恒化器培养中均产生了高水平的LDH。在需氧、葡萄糖受限的恒化器中，LDH表达不影响发生呼吸发酵代谢的稀释率（Dc）。高于Dc时，表达LDH的菌株同时产生乙醇和乳酸，但其总体发酵速率与野生型培养物相同。将进行呼吸作用的、表达LDH的培养物暴露于过量葡萄糖中会引发乙醇和乳酸的同时产生。然而，比葡萄糖消耗速率不受影响，这表明在这些条件下NADH的再氧化并不控制糖酵解通量。
