产朊假丝酵母ADE1基因的一级结构
Primary structure of the ADE1 gene from Candida utilis.
作者信息
Nishiya Y
机构信息
Tsuruga Institute of Biotechnology, Toyobo Co., Ltd., Fukui, Japan.
出版信息
Biosci Biotechnol Biochem. 1994 Jan;58(1):208-10. doi: 10.1271/bbb.58.208.
PMID:7764516
Abstract
The ADE1 gene from Candida utilis CA(u)-37, a strain used for commercially producing enzymes, was cloned by complementation of the ade1 mutation of Saccharomyces cerevisiae. It was composed of 903 bp, and the deduced amino acid sequence was 70% homologous to those of the ADE1 genes of S. cerevisiae and Candida maltosa. The highly preserved region of SAICAR synthetase, the ADE1 gene product, was also found by a homology search.
摘要
利用酿酒酵母ade1突变体的互补作用，克隆了用于商业生产酶的粟酒裂殖酵母CA(u)-37菌株的ADE1基因。该基因由903个碱基对组成，推导的氨基酸序列与酿酒酵母和麦芽糖假丝酵母的ADE1基因的氨基酸序列同源性为70%。通过同源性搜索还发现了ADE1基因产物SAICAR合成酶的高度保守区域。
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