麦芽糖假丝酵母ADE1基因的分子克隆

Molecular cloning of the Candida maltosa ADE1 gene.

作者信息

Sasnauskas K, Jomantiene R, Geneviciute E, Januska A, Lebedys J

机构信息

Department of Molecular Biology, Institute of Applied Enzymology, Vilnius, Lithuanian Republic.

出版信息

Gene. 1991 Oct 30;107(1):161-4. doi: 10.1016/0378-1119(91)90311-x.

DOI:10.1016/0378-1119(91)90311-x

PMID:1743515

Abstract

The structural gene (ADE1) encoding phosphoribosyl-aminoimidazole-succinocarboxamide synthetase (SAICAR synthetase; EC 6.3.2.6) in Candida maltosa has been isolated by functional complementation of an ade1 strain of Saccharomyces cerevisiae. The gene was localized on a 2.5-kb BamHI DNA fragment. Nucleotide sequence analysis of the cloned gene has revealed an open reading frame encoding a protein (SAICAR synthetase) with an Mr of 32,751. The codon bias index, 0.68, indicates that the ADE1 gene is a moderately highly expressed gene. The cloned gene shows 63.5% nt identity and 65.2% deduced amino acid identity with the S. cerevisiae ADE1 gene which encodes the same enzymatic activity. The gene may be used as a convenient genetic marker for construction of a new host-vector system for C. maltosa.

摘要

通过对酿酒酵母ade1菌株进行功能互补，已分离出麦芽糖假丝酵母中编码磷酸核糖氨基咪唑琥珀酰胺羧酰胺合成酶（SAICAR合成酶；EC 6.3.2.6）的结构基因（ADE1）。该基因定位在一个2.5kb的BamHI DNA片段上。对克隆基因的核苷酸序列分析揭示了一个编码蛋白质（SAICAR合成酶）的开放阅读框，其分子量为32751。密码子偏好指数为0.68，表明ADE1基因是一个中等高表达基因。克隆的基因与编码相同酶活性的酿酒酵母ADE1基因显示出63.5%的核苷酸同一性和65.2%的推导氨基酸同一性。该基因可作为构建麦芽糖假丝酵母新宿主-载体系统的便捷遗传标记。