Kondo Y, Toyoda A, Fukushi H, Yanase H, Tonomura K, Kawasaki H, Sakai T
Department of Agricultural Chemistry, Faculty of Agriculture, University of Osaka Prefecture, Japan.
Biosci Biotechnol Biochem. 1994 Mar;58(3):526-30. doi: 10.1271/bbb.58.526.
A 1.7-kb DNA fragment cloned from Zymomonas mobilis genomic DNA complemented the inability to grow on sucrose of a Suc- mutant of Z. mobilis that was deficient in the production of both extracellular levansucrase and invertase. Analysis of the nucleotide sequence of the fragment found two open reading frames (ORFs), both of which did not correspond to the structural gene for the levansucrase or the invertase. By subcloning each ORF into two different Suc- mutants of Z. mobilis, it has been found that the first ORF (gene zliE) activates the production of the extracellular levansucrase and invertase, and the second ORF (gene zliS) stimulates the secretion of the two enzymes. Gene zliS might contribute to the secretion of proteins having no signal peptide. The expression of zliE and zliS seemed to be under the control of the same promoter.
从运动发酵单胞菌基因组DNA中克隆出的一个1.7 kb DNA片段，弥补了运动发酵单胞菌蔗糖缺陷型突变体在蔗糖上无法生长的缺陷，该突变体缺乏细胞外果聚糖蔗糖酶和转化酶的产生。对该片段核苷酸序列的分析发现了两个开放阅读框（ORF），它们都与果聚糖蔗糖酶或转化酶的结构基因不对应。通过将每个ORF亚克隆到运动发酵单胞菌的两个不同蔗糖缺陷型突变体中，发现第一个ORF（基因zliE）激活细胞外果聚糖蔗糖酶和转化酶的产生，第二个ORF（基因zliS）刺激这两种酶的分泌。基因zliS可能有助于无信号肽蛋白质的分泌。zliE和zliS的表达似乎受同一启动子的控制。
